Open AccessFormation of Indoleacetic Acid by Enterbacteria Pathogenic for Human
Author(s) -
А. Л. Турская,
Л. А. Беловежец,
Tatiana Putilina,
В. А. Быбин,
А. В. Духанина,
Ю. А. Маркова
Publication year - 2019
Publication title -
acta biomedica scientifica
Language(s) - English
Resource type - Journals
eISSN - 2587-9596
pISSN - 2541-9420
DOI - 10.29413/abs.2019-4.5.2
Subject(s) - pectobacterium carotovorum , enterobacteriaceae , bacteria , pathogenic bacteria , biology , colonization , acetic acid bacteria , microbiology and biotechnology , salmonella , indole 3 acetic acid , microorganism , escherichia coli , auxin , biochemistry , gene , genetics
Background . At present, the problem of plant colonization by bacteria pathogenic for human is of particular interest. Among the bacteria capable of polyhostality, species of the Enterobacteriaceae family, which includes both phytopathogenic and human pathogens, are of particular interest. Fruits and vegetables contaminated with such pathogens have been shown to be able to cause human intestinal infections. Being one of the ways of communication in the plantmicroorganism system, it is possible that the synthesis of indole acetic acid (IAA) by Enterobacteriaceae determines the growth rate of bacteria, contributing to more rapid colonization of the plant. Aim : comparative study of the synthesis of IAA by different types of enterobacteria. Methods. Eight types of enterobacteria were used in the study, seven of which were isolated from sick people, as well as the phytopathogen Pectobacterium carotovorum spp. carotovorum. The work was performed using microbiological and spectrophotometric research methods. Results. The ability of the synthesis of IAA by opportunistic microorganisms belonging to the Enterobacteriaceae family was studied. We found that the majority of the studied strains synthesize IAA. Studies on the effect on the synthesis of IAA, the introduction into the nutrient medium of tryptophan and its enrichment with glucose, as well as the variation of the temperature regime of the cultivation of bacteria were carried out.