Open AccessINFLUENCE OF BRUCELLA ABORTUS I-206 THERMOEXTRACTS IN L- AND S-FORM ON MORPHOFUNCTIONAL STATE OF WHITE MICE ADRENAL GLANDS
Author(s) -
В. И. Дубровина,
Т. П. Старовойтова,
S. A. Vityazeva,
Н. Л. Баранникова,
Т. А. Иванова,
Т. Т. Шкаруба,
С. В. Балахонов
Publication year - 2018
Publication title -
acta biomedica scientifica
Language(s) - English
Resource type - Journals
eISSN - 2587-9596
pISSN - 2541-9420
DOI - 10.29413/abs.2018-3.4.15
Subject(s) - brucellosis , adrenal gland , brucella abortus , biology , peripheral blood , medicine , immunology , virology , endocrinology
Live vaccines are used for specific brucellosis prophylaxis in the Russian Federation. These vaccines in addition to a number of positive properties have some limitations including high agglutinogenicity, reactogenicity, sensitizing activity. In this connection, the development of subunit vaccines without adverse reactions is a perspective direction in modern vaccinology. Complex morphological research of the adrenal glands and comparative analysis of peripheral blood (leukogram, leukocytic index and index of allergization) of white mice immunized with thermoextracts (ТE) of Brucella abortus I-206 in L- and S-form and inactivated vaccine B. abortus 19 ВА were conducted. It was shown that ТE unlike B. abortus 19 ВА caused minor alterations in peripheral blood of the experimental animals in early periods of observation (increase of allergization index, changes in leukogram) with the subsequent levelling to the values in control. Expositions of the adrenal gland zoning were determined and cellular structure was estimated in consideration of morphometry. Changes in architectonics of the mice organ immunized with B. abortus 19 ВА were revealed. These alterations could indicate the stress-reaction development. In case of ТE application the given changes were insignificant and were developed in early periods. The revealed morphological changes in adrenal glands of laboratory animals permit to prove the necessity of realization the further experimental researches to ТE application as the components for development of a subcellular brucellosis vaccine.