Open AccessCytotoxic and Antitumoral Effects of Bikaverin from Gibberella fujikuroi on L5178Y Lymphoma Murine Model
Author(s) -
Gabriela Hinojosa Ventura,
Ana María Puebla Pérez,
Martha Patricia Gallegos Arreola,
Ma. del Carmen Chávez Parga,
Antonio Romero Estrada,
Jorge Iván Delgado Saucedo
Publication year - 2019
Publication title -
journal of the mexican chemical society
Language(s) - English
Resource type - Journals
ISSN - 2594-0317
DOI - 10.29356/jmcs.v63i4.729
Subject(s) - cytotoxic t cell , lymphoma , inoculation , ic50 , t cell lymphoma , cytotoxicity , chemistry , in vivo , cell culture , in vitro , microbiology and biotechnology , biology , immunology , biochemistry , genetics
In this work, bikaverin was isolated from the mycelium of Gibberella fujikuroi and characterized using 1D (1H and 13C) and 2D (HSQC and HMBC) NMR spectroscopy. Its cytotoxic effect on L5178Y lymphoma cells and antitumor effect on BALB/c mice inoculated with L5178Y cells were evaluated. According to the results, bikaverin showed cytotoxic effect against L5178Y lymphoma cells with an IC50 value of 0.23 μg/mL.The tumor evolution, with and without treatment, was observed in the in vivo study; the group without treatment showed 1,280±128 x 105 cells/mL in ascites volume, and the groups treated with bikaverin at the dose of 1.73 mg/kg/day by intraperitoneal (i.p.) and oral administration, decreased significantly the cell density, and showed 391.5±237 x 105 cells/mL and 580±208 x 105 cells/mL, respectively. The results confirm the cytotoxic effect of bikaverin on cancer cell lines and contribute to show the potential of bikaverin as antitumoral compound in BALB/c mice inoculated with L5178Y lymphoma cells.