Open AccessBehavior of Two and Three Electrode Configuration and Different Mediators in Working Electrode on Development of Disposable Screen-Printing Biosensors for Determination of Free Cholesterol
Author(s) -
Mónica Hernández-Cruz,
Carlos Andrés Galán-Vidal,
Giaan Arturo Álvarez-Romero,
Marı́a Teresa Ramı́rez-Silva,
María Elena Páez-Hernández,
José Luis González-Vidal
Publication year - 2017
Publication title -
journal of the mexican chemical society
Language(s) - English
Resource type - Journals
ISSN - 2594-0317
DOI - 10.29356/jmcs.v57i1.237
Subject(s) - biosensor , prussian blue , screen printing , amperometry , electrode , transducer , materials science , nanotechnology , working electrode , reproducibility , cholesterol oxidase , electrochemistry , chemistry , chromatography , cholesterol , electrical engineering , engineering , composite material , biochemistry
In the present work the development of an amperometric transducer in order to build a free cholesterol biosensor in planar configuration is reported. The one single use disposable biosensors were constructed by screen printing process which is compatible with automated methodologies of production. Based in the incorporation of tetracyanoquinodimethane (TCNQ), Prussian blue (PB) or ferrite (Fe 3 O 4 ) as mediator or electrocatalytic agent, three types of electrochemical transducers were evaluated. The cholesterol biosensors require a sample volume of 7.2 μL, exhibits good reproducibility and selectivity and cover a lineal answer of 2-16 mM with detection limits from 0.3 to 1.6 mM. The characteristics of biosensors are satisfactory for the decentralized analysis of the lipid in blood since enclose the range of clinical interest (3.5-6.5 mM).