Open AccessIdentification of Proteins from Cap-Binding Complexes by Mass Spectrometry During Maize (Zea mays L.) Germination
Author(s) -
Pedro E. Lázaro-Mixteco,
Tzvetanka D. Dinkova
Publication year - 2017
Publication title -
journal of the mexican chemical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.172
H-Index - 22
ISSN - 2594-0317
DOI - 10.29356/jmcs.v56i1.273
Subject(s) - germination , zea mays , hsp70 , heat shock protein , biology , chemistry , mass spectrometry , biochemistry , botany , chromatography , agronomy , gene
This work describes the identification of components in the Cap-binding complexes in non-germinated and 24-h-imbibed seeds using mass spectrometry. This approach revealed new components particularly present in the non-germinated seed. Among these, two heat shock proteins, HSP101 and HSP70, were detected as well as several proteins involved in carbohydrate metabolism. Between the new components of maize Cap-binding complexes, several proteins contain a motif that identifies them as potential direct interactors with eIF4E or eIF(iso)4E.Together with the major abundance of eIF(iso)4E at this developmental stage, our findings indicate clear differences between the translation initiation complexes that are available for protein synthesis right upon water imibition and those that are present once germination has been completed.