Open AccessAge-related changes in cell yield and viability of feline Adipose Tissue-Derived Mesenchymal Stem (fAD-MSCs)
Author(s) -
Nissar Shazia,
Geetha Ramesh,
Basha Sabiha Hayath,
Kannan TA,
Hussain Shahid
Publication year - 2022
Publication title -
journal of stem cell therapy and transplantation
Language(s) - English
Resource type - Journals
ISSN - 2577-1469
DOI - 10.29328/journal.jsctt.1001024
Subject(s) - trypan blue , mesenchymal stem cell , adipose tissue , collagenase , viability assay , stem cell , hemocytometer , andrology , cell , age groups , biology , medicine , chemistry , pathology , endocrinology , biochemistry , enzyme , microbiology and biotechnology , demography , sociology
In the present study, omental adipose tissue was collected from, the animals that underwent ovariectomy and ovariohysterectomy, surgical procedures, at the age of seven months to 11/2 years of age groups. The sample was digested with 0.1% (W/V) collagenase type I and transferred to a beaker with a magnetic stirrer and kept in a stirrer with 600 rpm at 37 °C for 30 minutes. The viability of the cell was evaluated by the trypan blue exclusion method using a hemocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the member in a dead cell and dye it blue. In the present study, the cell yield of fAD-MSCs was 8.15 ± 0.68, 6.55 ± 0.26, 4.85 ± 0.42, 3.90 ± 0.34, and 3.51 ± 0.43 in different age groups viz., 7,8,9 month 1 and 1½ year respectively. In younger age groups, cell yield and viability percentage were more than in animals of higher age groups. In the younger age group, stem cells proliferation status is considered potent for therapeutic application.