Open AccessExtraction of DNA from face mask recovered from a kidnapping scene
Author(s) -
Nsor Bassey,
Hajiya Mairo Inuwa
Publication year - 2022
Publication title -
journal of forensic science and research
Language(s) - English
Resource type - Journals
ISSN - 2575-0186
DOI - 10.29328/journal.jfsr.1001029
Subject(s) - dna extraction , dna , crime scene , saliva , chromatography , blood stains , extraction (chemistry) , tears , sweat , mucus , nucleic acid , biology , computational biology , chemistry , polymerase chain reaction , genetics , gene , biochemistry , ecology , psychology , paleontology , criminology , immunology
Deoxyribonucleic acid (DNA) extraction has considerably evolved since it was initially performed back in 1869. It is the first step required for many of the available downstream applications used in the field of molecular biology and forensic science. Blood samples is one of the main body fluid used to obtain DNA. This experiment used other body fluids such as saliva, sweat tears and mucus. There are many different protocols available to perform nucleic acid extraction on such samples. These methods vary from very basic manual protocols to more sophisticated methods included in automated DNA extraction protocols. This experiment used extraction kit (Zymo research). The DNA result from isolated saliva samples on the facemask range from 133.7, 213.6, 599.1 and 209.1 mg/ml. theoretically; such DNA is of much quantity and quality and can be used for forensic investigation when recovered from a crime scene. The DNA from isolated tears samples on the face mask ranges from 707.7, 202.5, 99.2, and 62.6 mg/ml. Theoretically, such DNA is of much quantity and quality and can be used for forensic investigation when recovered from a crime scene. The DNA from isolated tears samples on the face mask ranges from 615.3, 66.2, 78.5, and 68.2 mg/ml. theoretically, such DNA is of much quantity and quality and can be used for forensic investigation when recovered from a crime scene. Extracted DNA from saliva and sweat produced visible bands on agarose gel, mucous stain produce obscure band on agarose gel and the tears stain produce invisible bands. DNA from sweat satin, saliva stain, mucus stain and tears stain in face mask can be used as alternative for forensic investigation.