Open AccessCYTOMORPHOLOGICAL TRANSFORMATIONS IN YADK-04 CELLS DURING INTERACTION WITH PESTE DE PETITS RUMINANTS VIRUS
Author(s) -
Б. Л. Манин,
Л. В. Малахова,
А. Б. Сарбасов,
Н. В. Мороз
Publication year - 2019
Publication title -
veterinariâ segodnâ
Language(s) - English
Resource type - Journals
eISSN - 2658-6959
pISSN - 2304-196X
DOI - 10.29326/2304-196x-2019-2-29-41-45
Subject(s) - cytopathic effect , virus , cytoplasm , titer , biology , virology , peste des petits ruminants , reproduction , syncytium , lysis , cell culture , chemistry , microbiology and biotechnology , genetics , ecology
The paper presents experimental study results of the cytopathic effect of peste de petits ruminants virus on a goat gonad continuous cell line (YaDK-04). The interaction of peste de petits ruminants virus with cells at different stages of its reproduction was shown using a combination of phase-contrast and luminescent microscopy. It was found that at the initial stage of interaction (20–24 hours) the cells became rounded and de-adhered, and the monolayer was partially loosened. On day 2 post reproduction the most part of the culture monolayer affected by the virus began to destruct, and the cell nuclei were displaced to periphery. At the terminal stage (72 hours) the destruction of monolayer cells and cytoplasmic matrix, deformation and partial lysis of the nuclei and cytoplasm, aggregation of detritus occurred. At the final stage of reproduction (96 hours) the peste de petits ruminants virus diffused into the culture medium, the fluorescence in the yellow spectrum decreased significantly, but the virus titer reached 6.89 lg TCD50/cm3.