DENTIGEROUS CYSTS AND AMELOBLASTOMAS

Objectives: The aim of study was to observe the expression of MCM2 in dentigerouscyst and ameloblastoma. Introduction: Minichromosome maintenance protein (MCM2) maybe a structurally and functionally complicated replication moiety that synergizes with differentmolecular factors therefore regulate DNA synthesis. MCM proteins play a job in maintaininggenomic integrity and stop re-replication once per cell cycle. It’s absent from chromatin inquiescent cells however abundant in mitotically active cells so making it a helpful marker forcellular proliferation. Dentigerous cyst (DC) is the commonest biological process odontogeniccyst having high proliferative index that may lead to dysplastic changes and developmentof tumours. Ameloblastoma is uncommon, benign and regionally aggressive odontogenicneoplasm with high rate of repetition after surgery. Study design: It was a descriptive study anddesigned to work out the expression of MCM2 in DCs and ameloblastomas. Setting: Departmentof Morbid Anatomy and Histopathology/ Oral Pathology. Period: Six months. Material andmethods: Twenty-five patients presenting with DCs (n=12) and ameloblastomas (n=13) wereselected. Clinical and radiographical findings were recorded and biopsies were submitted forhistological diagnosis. MCM2 immunopositivity was assessed by immunohistochemistry in fourmicroscopic high power fields showing most range of immunopositive cells. Results: Mean agewas 26.5± 11.24 years and 42.07± 9.24 years whereas male to feminine magnitude relationwas 7:5 and 7:6 for DCs and ameloblastomas severally. Most of the patients (58.3%) of DCswere asymptomatic whereas 41.6% patients reportable with painful swelling. Comparing, allpatients with ameloblastomas conferred with painless swelling. Radiographically, all DCs wereunicystic radiolucent lesions. While 46.2% of ameloblastomas were unicystic whereas 53.8%were multicystic radiolucent lesions. Histologically, basal layer atypia was seen in 50% and 23%of DCs and ameloblastomas respectively. High MCM2 immunoreactivity was ascertained withinthe epithelial lining of the DCs and the neoplastic cells of ameloblastomas. Conclusion: MCM2expresses the higher proliferation index that might lead to neoplastic development in DCs whilelocal invasive and recurrence potential in ameloblastomas.