Open AccessDIABETIC ALBINO RATS
Author(s) -
Navaid Kazi,
Din Muhammad Shaikh,
Muhammad Ahmed Azmi
Publication year - 2016
Publication title -
the professional medical journal/the professional medical journal
Language(s) - English
Resource type - Journals
eISSN - 2071-7733
pISSN - 1024-8919
DOI - 10.29309/tpmj/2016.23.05.1591
Subject(s) - medicine , glutathione peroxidase , alloxan , cassia , diabetes mellitus , traditional medicine , analysis of variance , endocrinology , superoxide dismutase , oxidative stress , pathology , traditional chinese medicine , alternative medicine
Objectives: The present study evaluated the blood glucose, superoxidedismutase (SOD) and glutathione peroxidase (GPX) in Cinnamon cassia bark extract (CCBE)treated alloxan-induced diabetic albino rats. Study Design: Experimental study. Setting: Animalhouse of Al-Tibri Medical College, Karachi was selected for conducting the present study.Period: January 2014 to December 2014. Methodology: A sample of twenty rats was randomlydivided into 4 groups. Rats were grouped as A1 (n=5) – Negative control, A2 (n=5)- Positivecontrols (alloxan treated rats), and experimental groups B1 (n=5) and B2 (n=5) includeddiabetic rats which received 0.3 grams and 0.6 grams of CCBE respectively. Intraperitonealalloxan (120 mg /kg in 0.5 ml dissolved in acetate buffer) was used for induction of diabetes.C. cassia bark extract was prepared under standard protocol. Blood glucose (glucose oxidasemethod), SOD and GPX (Elisa kit) were detected from blood samples after 30 days. Data wasanalyzed on SPSS. Data was analyzed by one- way ANOVA and post-Hoc was used for groupcomparisons. Data was analyzed at 95% CI (p< 0.05). Result: Blood glucose, SOD and GPXshowed statistically significant differences (p<0.036). CCBE treated rats showed low glucoselevels and elevated SOD and GPX enzyme activity (p<0.05).