EVALUATION OF PARAOXONASE (PON) GLUTAMATE OXALOACETATE TRANSAMINASE (SGOT)

Objectives: This study was undertaken for the comparative evaluationof paraoxonase (PON) and Serum Glutamate Oxaloacetate Transaminase (SGOT) levels in male cardiac patients andnormal subjects and the evaluation of cardiac dysfunction by assaying the enzymes PON and SGOT. Study design:Prospective Period: Six months from June 2003 to Nov 2003. Setting: DHQ Hospital Faisalabad/ Department ofChemistry Agriculture University of Faisalabad. Method & Materials: The blood of the male cardiac patients as wellas control subjects was collected and serum was separated, that was used for the determination of PON and SGOTactivity. Twenty blood samples from male cardiac patients from DHQ Faisalabad was collected. At the same time 20other male subjects which were not suffering from any active cardiac disease were selected as control. Ante-cubitalvenous blood samples without anticoagulant were centrifuged to obtain the serum. Results: Paraoxonase is presentin the blood on the HDL and it can break down the oxidized LDL to non-harmful products therefore the activity of HDLand LDL was determined along with other two parameters. It was observed that the cardiac male patients had lowactivity of PON and HDL as compared to normal. Whereas activity of SGOT and LDL was lower in normal subjects ascompared to the cardiac male patients. The activity of PON and HDL (56.86±0.94 U/ml), (14.58±0.14 mg/dl) is lowerin cardiac male patients as compared to normal (241±0.85 U/ml), (46.66±1.10 mg/dl)respectively, while SGOT (219.0±2U/ml and LDL (177.3±4.73 mg/dl) was greater as compared to the control (24.78±2.32 U/ml) and (119.2±1.08 mg/dl)respectively. Conclusion: It is concluded that there was an inverse correlation between serum PON and SGOT.