Open AccessCharacterization of PVA-Enzyme Coated Indicator Electrodes GA coated again with PVC-KTpClPB-o-NPOE UV-Vis analysis, variable signal analysis, sensor sensitivity and SEM-EDS
Author(s) -
Abd Hakim S
Publication year - 2021
Publication title -
jurnal penelitian pendidikan ipa
Language(s) - English
Resource type - Journals
eISSN - 2460-2582
pISSN - 2407-795X
DOI - 10.29303/jppipa.v7ispecialissue.1248
Subject(s) - glutaraldehyde , urease , absorbance , potentiometric titration , chemistry , analyte , biosensor , electrode , chromatography , nuclear chemistry , urea , analytical chemistry (journal) , materials science , biochemistry
This study aims to characterize the phosphate buffer and urease enzymes through UV-Vis and SEM-EDS absorbance spectra using tungsten as an indicator electrode. The method used is a potentiometric biosensor with urease enzyme immobilization technique for urea analyte. A small detection range of 10-5-10-4M has been studied with PVA-enzyme coated indicator electrodes coated with PVC-KTpClPB. On this basis, the researchers increased the detection range by analyzing glutaraldehyde (GA) mixed with PVA-enzyme and o-NPOE mixed with PVC-KTpClPB. The best results of GA mixed PVA-enzyme at GA2.9% UV-Visible analysis. The best results were PVA-enzyme coated indicator electrodes coated with GA coated again with PVC-KTpClPB-o-NPOE SEM-EDS analysis on PVA-enzyme samples 3x coated with GA 1x and PVC-KTpClPB-o-NPOE 1x with o-NPOE variation of 61% and 66%.