Open AccessIDENTIFIKASI SENYAWA METABOLIT SEKUNDER PADA HASIL FRAKSINASI EKSTRAK Phaseolus vulgaris L. DENGAN METODE GAS CHROMATOGRAPHY-MASS SPECTROSCOPY (GC-MS)
Author(s) -
Khurriyatul Khair,
Yayuk Andayani,
Aliefman Hakim
Publication year - 2017
Publication title -
jurnal penelitian pendidikan ipa
Language(s) - English
Resource type - Journals
eISSN - 2460-2582
pISSN - 2407-795X
DOI - 10.29303/jppipa.v3i1.51
Subject(s) - chemistry , chromatography , fractionation , gas chromatography , ethyl acetate , mass spectrometry , thin layer chromatography , fraction (chemistry) , gas chromatography–mass spectrometry
This research was aimed to identify some classes of secondary metabolite compound on the fractionation results of green bean extract (P. vulgaris L.) using Gas Chromatography-Mass Spectroscopy (GC-MS) method. Green bean was extracted by methanol solvent. Fractionation of methanol extract used Vacuum Liquid Chromatography (VLC) with some variation of eluents such as n-hexane 100%; n-hexane: ethyl acetate (9 : 1 to 1 : 9); and ethyl acetate 100%, produced 11 major fractions. Based on Thin Layer Chromatography (TLC) analysis using DCM: MeOH (9.5: 0.5) as mobile phases, these 11 major fractions were classified based on their polarity such as non-polar, semi-polar and polar fraction. The results of identification by GC-MS spectrometer showed the presence of secondary metabolites such as monoterpenes in non-polar fraction; in semi-polar fraction was found terpenoids and steroids; in polar fraction was found monoterpenes, phenolic and steroid as the lowest percent area, that is less than 2%. Keyword: P.vulgaris L extract, fractionation, VLC, GC-MS