Open AccessPhenotypic and Genotypic Characterization of Beta-lactams Resistant Staphylococcus aureus Isolates from Bovine Mastitis and its Zoonotic Implications
Author(s) -
Ammara Khan
Publication year - 2020
Publication title -
pakistan veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.281
H-Index - 29
eISSN - 2074-7764
pISSN - 0253-8318
DOI - 10.29261/pakvetj/2020.065
Subject(s) - staphylococcus aureus , biology , cefoxitin , microbiology and biotechnology , mastitis , sccmec , ampicillin , penicillin , genotype , antibiotic resistance , gene , virology , antibiotics , genetics , methicillin resistant staphylococcus aureus , bacteria
Rise of antibiotic resistant Staphylococcus aureus is a higher risk and great concern to global health. Zoonotic transfer of such strains is well documented. Present study evaluated the presence of resistant S. aureus from mastitic Nilli Ravi buffaloes and nasal carriage of milkers. Phenotypic profile of S. aureus isolates was conducted against penicillin, ampicillin, and cefoxitin. PCR analysis revealed presence of blaZ gene and mecA gene from S. aureus isolated from milk and milkers samples. Sequence and phylogenetic analysis depicts the divergence of mecA gene originated from bovine and human but for the blaZ gene, no divergence was detected. The high degree of genetic relatedness among blaZ and mecA genes in bovine and human S. aureus isolates from same farm suggests the potential transfer of antimicrobial resistance genes between buffaloes and milkers, highlighting the importance of one-health approach to promote global health