Preclinical Assessment of Antiurolithiatic Activity of Mangifera indica Seeds on Ethylene Glycol Induced Urolithiasis in Rat Model

Mangifera indica seeds (MIS) are used traditionally for treating multiple ailments including urolithiasis. In current study, aqueous methanolic extract of MIS was examined for antiurolithiatic potential against Calcium oxalate (CaOx) crystals. In vitro analysis (nucleation, aggregation and growth assays) was performed with 20, 40, 60, 80 and 100 mg/mL concentrations of extract against standard drug (cystone). In-vivo CaOx crystals were induced by administration of drinking water comprising 0.75% v/v ethylene glycol (EG) and 1% w/v ammonium chloride (AC) for initial 3 days followed by intake of 0.75% v/v EG for next 25 days. Total 36 rats were allocated into 6 groups receiving vehicle, EG + AC, Cystone and extract (250, 500 and 1000 mg/kg) respectively. Urine and blood samples were collected for biochemical analysis. In in vitro analysis, 1000 mg/mL concentration significantly inhibited crystal formation when compared to standard. While MISE (500 and 1000 mg/kg) produced significant reduction in serum creatinine, BUN and uric acid levels while increased urine volume, Mg, pH and citrate levels of urine in MISE treated rats. The results give a scientific basis for its traditional claims