Open AccessIn vitro antimicrobial effect of different root canal sealers against oral pathogens
Author(s) -
Ali Monajemzadeh,
Sina Ahmadi,
Sajad Aslani,
Batool Sadeghi-Nejad
Publication year - 2017
Publication title -
current medical mycology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.369
H-Index - 6
eISSN - 2423-3439
pISSN - 2423-3420
DOI - 10.29252/cmm.3.2.7
Subject(s) - antimicrobial , root canal , streptococcus mutans , chemistry , candida albicans , microbiology and biotechnology , agar , lactobacillus casei , zinc oxide eugenol , minimum inhibitory concentration , corpus albicans , streptococcus salivarius , bacteria , agar diffusion test , eugenol , agar plate , food science , dentistry , biology , antibacterial activity , medicine , fermentation , genetics , organic chemistry
Background and Purpose: Root canal therapy is the primary method for the treatment of an infected pulp in modern dentistry. The main aim of endodontic treatment is the elimination of bacteria and their products from infected root canals. In this study, we attempted to investigate the antimicrobial activity of three root canal sealers against oral pathogens. Materials and Methods: The antimicrobial effectiveness of three endodontic sealers with different chemical compositions, namely resin (AH 26), zinc oxide eugenol (ZOE), and mineral trioxide aggregate (MTA), against Candida albican s, Streptococcus sanguis, Streptococcus salivarius, Streptococcus mutans , and Lactobacillus casei was assayed by agar well diffusion method (AWDM). The tested sealers were prepared according to the manufacturer’s instructions and poured in the prepared wells of agar plates; diluted inocula (10 5 and 10 6 CFU/ml) of the tested microorganism strains were also used. The minimum inhibitory concentration (MIC) values of the selected canal sealers ranged between 3.12 and 50 mg.ml -1 against the employed microorganism strains. All the plates were incubated at 37°C under anaerobic condition for bacteria and at 30°C for C. albicans. After three days, the inhibition zones were measured.Results: In this investigation, AH 26 exhibited strong activity against C. albicans with the minimum inhibitory concentration of 12.5 mg.ml -1 , but ZOE and MTA did not act against C. albicans . ZOE sealer had the highest antimicrobial activity against the tested bacteria, while MTA showed the lowest antimicrobial activity.Conclusion: The ascending sequence of microbial growth inhibition zones was as follows AH 26 > ZOE > MTA.