Open AccessAbility of Bacteria Isolated from Black Water Ecosystem of Central Kalimantan in Degrading of Crude Oil and Diesel Oil
Author(s) -
Iswandi Anas,
Noegroho Hadi,
Dwi Andreas Santosa
Publication year - 2001
Publication title -
jurnal ilmu tanah dan lingkungan/jurnal ilmu tanah dan lingkungan (journal of soil science and environment)
Language(s) - English
Resource type - Journals
eISSN - 2549-2853
pISSN - 1410-7333
DOI - 10.29244/jitl.2.2.1-7
Subject(s) - bacteria , diesel fuel , incubation , food science , chemistry , biology , botany , environmental science , biochemistry , organic chemistry , genetics
The objective of this experiment was to evaluate the abili!y of bacteria strains isolated from black water ecosystem of Central Kalimantan in degrading crude oil and diesel oil. The experiment was conducted at the Laboratory of Soil Biology, Department of Soil Sciences, Faculty ~f Agriculture, BogorAgricultural University (IPB). The crude oil degrading ability of bacteria was tested on a minimum liquid medium (Gurujeyalakshmi dan Orial, 1989) and soil. Six crude oil and diesel oil degrading bacteria were isolated. The bacteria were identified as Bacillus panthotenticus (2 strains), B. circulars (1 strain), Pssudomonas diminuta (1 strain), P. stufzeri (1strain) and Klebsiella edwardsii (1 strain). These bacteria were able to degrade crude oil or diesel oil whether in a liquid minimum medium or in soil.On a minimum liquid medium, after 10 days incubation, bacteria were able to degrade 20.49% of the crude oil, while in the Entisol soil, after 7 days of incubation, as high as 40.29% of crude oil was degraded and after 28 days incubation, 64.95 of crude oil had been degraded by selected bacteria.