Open AccessIdentifikasi Keragaman Gen TGF-β2 dan Asosiasinya dengan Sifat Pertumbuhan pada Ayam Tolaki
Author(s) -
Muh Akramullah,
Cece Sumantri,
Niken Ulupi,
Muhammad Amrullah Pagala
Publication year - 2020
Publication title -
jurnal ilmu produksi dan teknologi hasil peternakan
Language(s) - English
Resource type - Journals
eISSN - 2615-594X
pISSN - 2303-2227
DOI - 10.29244/jipthp.8.1.22-29
Subject(s) - weight gain , genotype , single nucleotide polymorphism , biology , body weight , gene , feed conversion ratio , snp , allele , polymorphism (computer science) , transforming growth factor , zoology , genetics , endocrinology
The transforming growth factor-beta 2 (TGF-β2) gene is a group of growth hormone (GH) genes that control the nature of growth and belongs to the cytokine group. This study aimed to identify Single Nucleotide Polymorphism (SNP) g.640 T>C from the TGF-β2 gene then associated with the growth properties of tolaki chickens. Chicken blood samples used in this study amounted to 70 birds. Data on the nature of growth observed include; hatched weight, feed consumption, body weight, body weight gain and feed conversion. The TGF-β2 gene polymorphism was identified using the PCR-RFLP method. SNP g.640 T>C in the TGF-β2 gene was polymorphic and wasin Hardy-Weinberg balance. Identification results found 2 alleles, namely T and C and 3 genotype namely TT, TC, and CC. The association of TGF-β2 gene diversity in tolaki chicken generally showed a difference (P<0.05) on feed consumption, body weight and body weight gain. CC genotype has higher feed consumption, body weight, weight gain and more efficient feed conversion than TC and TT genotypes.