Open AccessIdentification of molecular markers for type 2 Diabetes mellitus in Sidoarjo, Indonesia
Author(s) -
Miftahul Mushlih,
Fitri Kumala Sari,
Hindah Sabrina Amin,
Siti Asriani Iknan
Publication year - 2020
Publication title -
jurnal teknologi laboratorium/jurnal teknologi laboratorium
Language(s) - English
Resource type - Journals
eISSN - 2580-0191
pISSN - 2338-5634
DOI - 10.29238/teknolabjournal.v9i2.235
Subject(s) - rapd , primer (cosmetics) , genetics , biology , polymorphism (computer science) , type 2 diabetes mellitus , type 2 diabetes , computational biology , diabetes mellitus , medicine , endocrinology , chemistry , gene , genotype , genetic diversity , environmental health , population , organic chemistry
T2DM can be triggered by two collaborating factors, namely genetics and the environment. This study aimed to identify genetic markers that can be used to detect the possibility of a person having T2D using the random amplified polymorphism DNA (RAPD) method. The study was carried out cross-sectional and involved 60 samples consisting of 30 positive T2D samples and 30 negative samples T2D. The primer used for PCR-RAPD was D20 (5'-ACCCGGTCAC-3’). The PCR-RAPD results were then analyzed using the scoring method and analyzed using the non-parametric Chi-Square test (cl: 95%). Among T2D, 576 bp band were confirmed to be markers in the patients.