Open AccessEvaluation of in vitro immunomodulatory properties of biologically active substances and drugs
Author(s) -
Еlena V. Duzh,
А. Е. Гончаров
Publication year - 2019
Publication title -
vescì nacyânalʹnaj akadèmìì navuk belarusì. seryâ medycynskìh navuk
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.138
H-Index - 1
eISSN - 2524-2350
pISSN - 1814-6023
DOI - 10.29235/1814-6023-2019-16-2-234-243
Subject(s) - jurkat cells , cd80 , apoptosis , in vitro , reactive oxygen species , tumor necrosis factor alpha , phagocytosis , biological activity , cell culture , chemistry , microbiology and biotechnology , immunology , biology , immune system , biochemistry , t cell , cytotoxic t cell , cd40 , genetics
A new combined method for screening of immunomodulatory properties of drugs was developed, including: expression of CD80, HLA-DR, CD32, CD205 and CD197, interleukin-12 production and apoptosis by DCs; production of reactive oxygen species and evaluation of phagocytosis by neutrophils; expression of CD69 and production of tumor necrosis factor-α by Jurkat-tat cells; and CD80 and HLA-DR expression, viability and apoptosis using the Daudi cell line.