Open AccessDevelopment of a multiplex system for determining 11 genetic markers of predisposition to obesity
Author(s) -
Galina Sergeev,
Irina Haidukevich,
A. M. Gorkavaya,
Maxim Ameliyanovich,
Irma Mosse,
Sergey A. Usanov
Publication year - 2021
Publication title -
vescì nacyânalʹnaj akadèmìì navuk belarusì. seryâ hìmìčnyh navuk
Language(s) - English
Resource type - Journals
eISSN - 2524-2342
pISSN - 1561-8331
DOI - 10.29235/1561-8331-2021-57-3-300-309
Subject(s) - multiplex , genetics , snapshot (computer storage) , biology , gene , genetic predisposition , multiplex polymerase chain reaction , polymerase chain reaction , computer science , operating system
A system has been developed to identify 11 genetic markers associated with the risk of obesity: rs10852521, rs11075990, rs1121980, rs1421085, rs1477196, rs17817449, rs3751812, rs7206790, rs8047395, rs9940128 (FTO gene) and rs1137101 (LEPR gene) by minisequencing (SNaPshot analysis). The conditions for carrying out the amplification and minisequencing reactions, as well as the compositions of the reaction mixtures, were optimized so that the analysis was carried out for all 11 markers simultaneously. The resulting system was tested and showed a high degree of reproducibility and sensitivity required for the detection of these polymorphisms.