Open AccessHydrolysis of UV-induced peroxidized phosphatidylcholine initiated by phospholipases of different substrate specificities
Author(s) -
Н. М. Литвинко
Publication year - 2021
Publication title -
vescì nacyânalʹnaj akadèmìì navuk belarusì. seryâ hìmìčnyh navuk
Language(s) - English
Resource type - Journals
eISSN - 2524-2342
pISSN - 1561-8331
DOI - 10.29235/1561-8331-2021-57-2-195-205
Subject(s) - phosphatidylcholine , micelle , substrate (aquarium) , dynamic light scattering , chemistry , irradiation , liposome , phospholipase a2 , phospholipase , supramolecular chemistry , biophysics , chromatography , biochemistry , membrane , organic chemistry , enzyme , phospholipid , materials science , nanoparticle , biology , nanotechnology , molecule , ecology , physics , aqueous solution , nuclear physics
The activity of porcine pancreatic phospholipase A2 and the same of cobra venom toward phosphatidylcholine having different supramolecular organization and interfacial charge (micelles with sodium deoxycholate and liposomes) under UV irradiation (180–400 nm) was studied. It was shown that the UV-irradiated lipid phase is characterized by an increased index of phosphatidylcholine oxidation and the absence of a peak with a maximum of 235.5 nm, related to the presence of unsaturated bonds in the UV spectrum of docosahexaenoic acid, but retained in the presence of the antioxidant trolox. The activation of both phospholipases A 2 after UV irradiation of the substrate was established, regardless of its supramolecular organization, the charge of the interfacial surface, and the substrate specificity of the enzymes. Using dynamic light scattering, 0.3 % of larger particles were found among the irradiated micelles of phosphatidylcholine. The results obtained indicate that areas of accumulation of hydroperoxidized lipids can be formed in the irradiated model membrane, which serve as a site of intensified attack for phospholipases.