Open AccessENZYME IMMUNOASSAY KIT FOR THE DETERMINATION OF DEOXYNIVALENOL
Author(s) -
И. И. Вашкевич,
О. С. Куприенко,
I. V. Gorbachova,
A. A. Yastrebova,
T. V. Terentieva,
G. S. Kornilovich,
L. N. Sukhenko,
A. I. Shibeko,
О. В. Свиридов
Publication year - 2018
Publication title -
vescì nacyânalʹnaj akadèmìì navuk belarusì. seryâ hìmìčnyh navuk
Language(s) - English
Resource type - Journals
eISSN - 2524-2342
pISSN - 1561-8331
DOI - 10.29235/1561-8331-2018-54-3-319-328
Subject(s) - immunoassay , conjugate , chromatography , mycotoxin , polyclonal antibodies , horseradish peroxidase , detection limit , chemistry , bovine serum albumin , coefficient of variation , enzyme , food science , antibody , biochemistry , biology , mathematics , immunology , mathematical analysis
A reagent kit EIA-DEOXYNIVALENOL for the determination of mycotoxin deoxynivalenol (DON) in feeds and foods by a direct competitive enzyme immunoassay using microtitration plate has been developed and tested. The basic components of the kit are polyclonal antibodies to DON, obtained as a result of immunization of rabbits with a conjugate of DON with bovine serum albumin and a conjugate of horseradish peroxidase with DON. The evaluated parameters of the kit and metrological characteristics of the technique of measurements correspond to the modern level of immunoassay development and provide the determination of DON content of agricultural products in a range of 0.2 to 6.0 mg/kg with proper accuracy and precision. The limit of quantitative determination of DON in grain and cereal foods does not exceed 0.2 mg/kg.