Open AccessSome metal binding properties of recombinant human lactoferrin from the milk of transgenic goats
Author(s) -
Denis A. Semenov,
О. С. Куприенко,
И. И. Вашкевич,
О. В. Свиридов
Publication year - 2022
Publication title -
doklady nacionalʹnoj akademii nauk belarusi
Language(s) - English
Resource type - Journals
eISSN - 2524-2431
pISSN - 1561-8323
DOI - 10.29235/1561-8323-2022-66-1-43-54
Subject(s) - lactoferrin , recombinant dna , chemistry , ferric , europium , chelation , metal ions in aqueous solution , fluorescence , lanthanide , mass spectrometry , metal , fluorescence spectroscopy , nuclear chemistry , transferrin , chromatography , biochemistry , ion , inorganic chemistry , organic chemistry , physics , quantum mechanics , gene
In this research, the ability of pure recombinant human lactoferrin (rhLF), originated from the milk of transgenic goats, to bind ferric and europium ions has been shown by the methods of spectrophotometry, fluorescent spectroscopy, and inductively coupled plasma mass spectrometry. The apo-form of rhLF and its complexes with Fe 3+ or Eu 3+ saturated in 76 or 22 %, respectively, were obtained. A method for detection of total or released (“free”) lanthanide at acidic or neutral pH and high or low concentrations of chelating agents by time-resolved fluorescence was proposed.