Open AccessAnalysis of the cellular composition of the native auto-transplantation mixture used for plastics of bone tissue defects
Author(s) -
Н. В. Чуешова,
И. А. Чешик,
Э. А. Надыров,
В. И. Николаев,
С. И. Кириленко,
В. В. Рожин,
А. Н. Кондрачук,
N. S. Serdyuchenko
Publication year - 2021
Publication title -
doklady nacionalʹnoj akademii nauk belarusi
Language(s) - English
Resource type - Journals
eISSN - 2524-2431
pISSN - 1561-8323
DOI - 10.29235/1561-8323-2021-65-6-715-723
Subject(s) - immunophenotyping , fibrin , cd90 , flow cytometry , chemistry , population , bone healing , bone marrow , pathology , transplantation , cell , medicine , immunology , anatomy , cd44 , surgery , biochemistry , environmental health
The cell composition of native transplant autosmes (NTA) used for bone plastics was studied. The histological examination showed the fragments of bone beams with preserved osteoblasts, the foci of myeloid and lymphoid hematopoiesis and the fibrin deposits, which suggested the presence of MMSCs. Immunophenotyping of the NTA cell population revealed a high level of expression of the surface markers CD105, CD73, and CD90 characteristic for MMSC. DNA-flow cytometry of the bone dust confirmed almost complete preservation of graft viability on the 3rd day of culturing (97.7 % of live cells). The data of this study confirm the presence of the osteogenic, osteoinductive, and osteoconductive properties of the bone dust and emphasize the importance of a further study of this-type bone graft for use in surgical interventions.