Open AccessDetermining the human chronological age from the blood samples on the basis of the analysis of CpG-dinucleotides methylation
Author(s) -
В. А. Лемеш,
V. N. Kipen,
M. V. Bahdanava,
А. А. Буракова,
A. A. Bulgak,
A. V. Bayda,
V. V. Zotova,
V. I. Dobysh
Publication year - 2021
Publication title -
doklady nacionalʹnoj akademii nauk belarusi
Language(s) - English
Resource type - Journals
eISSN - 2524-2431
pISSN - 1561-8323
DOI - 10.29235/1561-8323-2021-65-5-582-591
Subject(s) - cpg site , dna methylation , methylation , human blood , computational biology , human genome , biology , biological age , correlation , evolutionary biology , genome , statistics , genetics , dna , mathematics , gene , physiology , gene expression , geometry
Based on the bioinformatic and statistical analysis of the GEO-projects to determine the genome-wide profile of human DNA methylation, a list of 27 CpG dinucleotides with a high predictive potential was formed to create models for prediction of the human age from blood samples. The methylation level was determined for 245 samples of individuals from the Republic of Belarus. The correlation coefficients R were calculated, and the mathematical models for determining the age of an individual were constructed. The average accuracy value of the age prediction from blood samples using 12 CpG-dinucleotides was 3.4 years (for men – 3.3, for women – 3.5). The results obtained will be used as a basis for development of calculators for predicting the age of an individual based on the biological traces for forensic experts.