Open AccessEngineering a whole rat liver scaffold
Author(s) -
Andrei D. Dubko,
M. Yurkevich,
M. Labai,
A. V. Svirskaya,
N. G. Yukhanov,
Tatsiana Savitskaya,
Darya Nizheharodava,
M. M. Zafranskaya
Publication year - 2021
Publication title -
doklady nacionalʹnoj akademii nauk belarusi
Language(s) - English
Resource type - Journals
eISSN - 2524-2431
pISSN - 1561-8323
DOI - 10.29235/1561-8323-2021-65-4-448-455
Subject(s) - decellularization , scaffold , tissue engineering , biomedical engineering , liver tissue , perfusion , sodium dodecyl sulfate , chemistry , matrix (chemical analysis) , biochemistry , medicine , chromatography
Engineering a three-dimensional scaffold opens up great prospects for creation of manufacturing biological artificial organs. The article presents a method of perfusion decellularization of a rat liver, with the main problems and options for their solution being analyzed. Perfusion of a donor liver with 0.1 % a sodium dodecyl sulfate (SDS) solution allows obtaining a high-quality cell-free matrix characterized by preserved hepatic architectonics, patent vascular bed, residual DNA of less than 1 %, no signs of collagen fibers destruction and tissue edema. The obtained scaffold can be used for recellularization by allogeneic cell cultures when creating volumetric tissue-engineered designs.