Open AccessAnalysis of the intralinear polymorphism and the homozygosity rate of triticale doubled haploid lines by microsatellite markers
Author(s) -
Е. В. Лагуновская,
А. А. Буракова,
В. Н. Буштевич,
В. И. Сакович,
В. А. Лемеш,
С. И. Гриб
Publication year - 2020
Publication title -
doklady nacionalʹnoj akademii nauk belarusi
Language(s) - English
Resource type - Journals
eISSN - 2524-2431
pISSN - 1561-8323
DOI - 10.29235/1561-8323-2020-64-2-199-208
Subject(s) - doubled haploidy , triticale , biology , microsatellite , ploidy , genetics , locus (genetics) , hybrid , polymorphism (computer science) , allele , botany , gene
We evaluated the rate of polymorphism of doubled haploid lines of hexaploid triticale obtained by the method of anther culture based on hybrids of spring and winter types. Using 7 SSR markers for the loci on the chromosomes A- ( Xgwm186, Xgwm291, Xgwm595 ) and B- ( Xgwm371, Xgwm540, Xgwm554, Xgwm234 ), polymorphism of 38 doubled haploid lines of hexaploid triticale was studied. Interlinear polymorphism along six microsatellite loci except the Xgwm554 locus, which is not polymorphic in the studied doubled haploid lines, was revealed. The highest polymorphism was observed for the Xgwm186 , Xgwm291 and Xgwm595 loci. The cluster analysis showed that all studied lines were divided into three main groups. The origin of the lines did not affect the distribution in groups. This confirms the influence of in vitro culture somaclonal variation. Eight lines of doubled haploids, which are heterozygous for one of the studied microsatellite loci, were identified. We showed the possibility of using SSR markers to assess interlinear polymorphism and the homozygosity rate in the triticale doubled haploid lines obtained by the method of induced androgenesis in vitro .