Open AccessScreening, characterization and molecular-genetical identification of a new bacterial strain Paenibacillus species
Author(s) -
Л. И. Сапунова,
А. Г. Лобанок,
К. К. Яцевич,
С. А. Кулиш,
И. О. Тамкович,
Л. В. Ерхова,
Я. Н. Сысолятин
Publication year - 2019
Publication title -
doklady nacionalʹnoj akademii nauk belarusi
Language(s) - English
Resource type - Journals
eISSN - 2524-2431
pISSN - 1561-8323
DOI - 10.29235/1561-8323-2019-63-2-181-188
Subject(s) - paenibacillus , biology , cellulase , 16s ribosomal rna , microbiology and biotechnology , polysaccharide , biochemistry , enzyme , gene
Bacterial variant PS-K-17 was isolated from wheat grain contaminated by polysaccharide-producing microbiota for further characterization. It was found that the isolate grown on agar slants and in submerged culture on media with specific substrates synthesized beta-galactosidase, amylase, protease, pectinase, cellulase, beta-glucanase, lipase (esterase), alginase, extracellular polysaccharides, and pigments, probably carotenoids. Based on cultural-morphological and physiological-biochemical properties and phylogenetic analysis of nucleotide sequences of 16S rRNA gene (access code MF443394 in GenBank) the bacterial culture was identified as Paenibacillus species PS-K-17. The studied isolate forms one phylogenetic branch with type strains Paenibacillus nicotianae (98.3 %), Paenibacillus hordei (98.2 %), Paenibacillus kyungheensis (97.9 %), holding wherein a separate position. Strain Paenibacillus sp. PS-K-17 may find use in biotechnology as a producer of extracellular polysaccharides and enzymes splitting plant polymeric substances as well as a component of microbial consortium-ingredient of a new complex feed additive.