Open AccessAnalysis of the genome of the bacteria Bacillus amyloliquefaciens BIM B-439D
Author(s) -
М. А. Титок,
Л. Н. Валентович,
А. В. Бережная,
Э. И. Коломиец
Publication year - 2018
Publication title -
doklady nacionalʹnoj akademii nauk belarusi
Language(s) - English
Resource type - Journals
eISSN - 2524-2431
pISSN - 1561-8323
DOI - 10.29235/1561-8323-2018-62-5-592-600
Subject(s) - prophage , surfactin , biology , genome , genetics , polyketide , bacillus amyloliquefaciens , bacteriocin , computational biology , bacillus subtilis , bacteria , gene , bacteriophage , escherichia coli , biosynthesis
According to the results of the analysis of the complete nucleotide sequence of B. amyloliquefaciens subsp. plantarum BIM B-439D, it is established that the genome of the strain is unique and is represented by a single ring chromosome with a size of 3978134 base pair containing 46.5 % G/C-pairs. The key genetic loci determining synthesis of antimicro-bic metabolites are defined: lipopeptides (surfactin, fengycin, bacillomycin D), dipeptide (bacilysin), siderophor (bacillibac-tin), polyketide antibiotics (difficidin and oxydifficidin, bacillaene and macrolactin), bacteriocin (amylocyclicin) and peptide/ polyketide (putative - tyrocidin), restriction-modification systems and mobile genetic elements (IS-elements and prophages) are characterized. The identified features in the organization and localization of individual genetic determinants (for example, intact prophage of 37558 bp) can be used as reliable molecular genetic markers for fast identification of the strain when it is used commercially. The complete nucleotide sequence of the genome can serve as the basis for a detailed functional analysis of the practically significant properties of the microorganisms of the Bacillus group.