Open AccessPolyvinyl alcohol magnetic microspheres containing 2-aminopyrimidin-bis(ethene)bis(dialkylphenylamine) compounds as fluorescent pH indicators
Author(s) -
К. В. Лазнев,
Ж. В. Игнатович,
I. N. Kukhta,
A. V. Agabekov
Publication year - 2018
Publication title -
doklady nacionalʹnoj akademii nauk belarusi
Language(s) - English
Resource type - Journals
eISSN - 2524-2431
pISSN - 1561-8323
DOI - 10.29235/1561-8323-2018-62-5-563-568
Subject(s) - glutaraldehyde , polyvinyl alcohol , microsphere , fluorescence , nuclear chemistry , chemistry , chromatography , organic chemistry , chemical engineering , quantum mechanics , engineering , physics
The fluorescent dyes 4,4′-(1E,1′Е)-2,2′-(2-aminopyrimidin-4,6-diyl) bis(ethene-2,l-diyl) bis(N,N-dimethylphenylamine) (АРМ) and 4′-(1E,1′Е)-2,2′-(2-aminopyrimidin-4,6-diyl) bis(ethene-2,l-diyl) bis(N,N-diethylphenylamine) (APE) were incorporated into glutaraldehyde-crosslinked polyvinyl alcohol microspheres containing magnetite. Fluorescence spectra of the obtained microspheres depend on pH of the medium. A technique for pH measurement within the range of 4–9 based on calculation of the green/red ratio of the fragments of color microimages of the microspheres was developed. only negligible loss of the fluorochromes from the microspheres occured during the storage time of 180 days. Thus the microspheres may be used as local pH indicators, e. g. for phagocytosis studies.