Open AccessIsolation and identification of microorganism’s resistant to soil salinization
Author(s) -
N. I. Naumovich,
Z. M. Aleschenkova,
I. N. Ananyeva,
H. V. Safronava
Publication year - 2022
Publication title -
vescì nacyânalʹnaj akadèmìì navuk belarusì. seryâ bìâlagìčnyh navuk
Language(s) - English
Resource type - Journals
eISSN - 2524-230X
pISSN - 1029-8940
DOI - 10.29235/1029-8940-2022-67-1-54-65
Subject(s) - halotolerance , bacillus megaterium , 16s ribosomal rna , microorganism , bacteria , chemistry , microbiology and biotechnology , rhodococcus , biology , food science , biochemistry , gene , genetics
315 variants of halotolerant bacteria were isolated from soil samples taken around Starobin potash deposit mined by Belaruskali concern. Nitrogen-fixing isolate CA-6 and phosphate-solubilizing isolate Cp-1 capable to withstand abiotic stress caused by sodium chloride in concentration 1710 and 2565 mM, respectively and water deficiency characterized by osmotic potential -0.20 and -0.42 MPa were sorted out. Isolate Cp-1 may dissolve phosphates yielding halo zones 9 mm in diameter while isolate CA-6 displays nitrogenase activity 44.0 nM C 2 H 2 /vial/3 days. Both isolates are growth-promoting organisms, distinguished by the ability to synthesize IAA in concentrations 89.7 and 37.8 μg IAA/ml of cultural liquid, respectively. The selected salt-resistant isolates were identified based on analysis of the nucleotide sequence of the 16S rRNA gene and data of MALDI-TOF mass spectrometry and were referred to the species Rhodococcus jostii and Priestia megaterium . Halotolerant strains Priestia megaterium Cp-1 and Rhodococcus jostii CA-6 were deposited in Belarusian collection of non-pathogenic microorganisms under the respective registration numbers BIM B-1314D and BIM B-1353D.