Open AccessChromatographic purification of the enzyme preparation from the cultural liquid of Pleurotus ostreatus
Author(s) -
V. V. Sakovich,
A. M. Hrusha,
V. V. Revenko,
D. D. Zhernossekov
Publication year - 2019
Publication title -
vescì nacyânalʹnaj akadèmìì navuk belarusì. seryâ bìâlagìčnyh navuk
Language(s) - English
Resource type - Journals
eISSN - 2524-230X
pISSN - 1029-8940
DOI - 10.29235/1029-8940-2019-64-4-467-471
Subject(s) - chromatography , pleurotus ostreatus , chemistry , sepharose , salting out , enzyme , ion chromatography , ion exchange , fast protein liquid chromatography , high performance liquid chromatography , biochemistry , food science , organic chemistry , ion , mushroom , aqueous solution
The primary purification of the proteolytic enzyme preparation from the Pleurotus ostreatus culture fluid was carried out. Methods of salting out, dialysis, ion-exchange chromatography on DEAEand KM-sepharose were used. It was shown that during the chromatography on KM-sepharose, the enzyme preparation from the culture liquid was divided into three fractions, one of which possessed milk-clotting activity (МСА). Chromatography on DEAE-sepharose allowed us to reach the significant purity of the enzyme with МСА.