Open AccessDesign of genetic construction for creation DNA vaccine against porcine reproductive and respiratory syndrome
Author(s) -
Л. М. Кравченко,
Kirill V. Kudzin,
U. A. Prakulevich
Publication year - 2018
Publication title -
vescì nacyânalʹnaj akadèmìì navuk belarusì. seryâ bìâlagìčnyh navuk
Language(s) - English
Resource type - Journals
eISSN - 2524-230X
pISSN - 1029-8940
DOI - 10.29235/1029-8940-2018-63-4-419-425
Subject(s) - porcine reproductive and respiratory syndrome virus , biology , virology , dna vaccination , immune system , gene , virus , plasmid , open reading frame , antigen , viral vector , vector (molecular biology) , immunology , genetics , recombinant dna , peptide sequence
The porcine reproductive and respiratory syndrome (PRRS) caused the serious economic damage to swine breeding around the world. It is a viral infective disease against which live attenuated and inactivated vaccines are not always successful. Development of new types of drugs such as DNA vaccines is necessary for improving the protection against the virus. DNA vaccines induce the development of both a cellular and humoral immune response. Such vaccines consist of a plasmid or viral vector with genes of potentially immunogenic proteins. The expression of these genes realized in cells of the vaccinated animal. It leads to the synthesis of antigen proteins triggering the immune response. The purpose of this work is to create a genetic construction that can be used as DNA vaccine against PRRS virus. The construction consists of the commercial vector pVAX1 and open reading frame of two structural proteins of PRRS virus, a lysosomal localization signal sequence of the invariant chain gene and regulatory elements necessary for the expression of cloned genes in mammalian cells.