Open AccessEstablishing CRISPR/Cas9 in Lipomyces starkeyi
Author(s) -
Zoe Lau,
David A. Stuart,
Bonnie A McNeil
Publication year - 2019
Publication title -
alberta academic review
Language(s) - English
Resource type - Journals
eISSN - 2561-5335
pISSN - 2561-5327
DOI - 10.29173/aar61
Subject(s) - yarrowia , crispr , yeast , genome editing , cas9 , biology , plasmid , saccharomyces cerevisiae , computational biology , biochemistry , gene
The goal of this project was to adapt the Yarrowia lipolytica plasmid based CRISPR/Cas9 system for usage in Lipomyces starkeyi. Lipomyces starkeyi is an oleaginous yeast, which synthesizes and stores high amounts of intracellular lipids. This specific yeast can store lipids at concentrations higher than 60% of its dry cell weight. Due to these high concentrations of lipids, L. starkeyi is a desired organism for the production of biofuels and other oleochemicals. However, there is a lack of knowledge and of genetic tools when trying to engineer the cells to produce these lipids for our use. The genome editing tool, CRISPR/Cas9 is efficient and simple, therefore desirable for the engineering of L. starkeyi. The goal was achieved by replacing the Y. lipolytica promoter with a L. starkeyi promoter, inserting guide RNA, as well as confirming cas9 protein expression.