Open AccessCOMPARATIVE EFFECTS OF BORIC ACID AND RESVERATROL ON MCF-7 BREAST CANCER CELLS METASTATIC BEHAVIOUR
Author(s) -
Tuğçe Aladağ,
Fatma Fırat
Publication year - 2022
Publication title -
international journal of research - granthaalayah
Language(s) - English
Resource type - Journals
eISSN - 2394-3629
pISSN - 2350-0530
DOI - 10.29121/granthaalayah.v10.i1.2022.4460
Subject(s) - vimentin , boric acid , epithelial–mesenchymal transition , cell migration , mcf 7 , metastasis , chemistry , mesenchymal stem cell , cancer research , resveratrol , cadherin , in vitro , cancer cell , cancer , cell , immunohistochemistry , biology , medicine , human breast , pathology , biochemistry , organic chemistry
Motivation/Background:The metastasis of tumor cells consists of steps such as epithelial-mesenchymal transition, transendothelial migration and the formation of metastatic colonization. E-Cadherin and vimentin are main proteins associated with EMT, whereas MMP-9 is associated with migration.
Method:We aimed to investigate effects boric acid and resveratrol comparatively on metaatatic behaviors on MCF-7. 30μM boric acid and 50μM resveratrol were administered to BA, BA+RES, and RES groups 48hours. Cells stained immunocytochemically by Anti-E-Cadherin, Anti-Vimentin, and Anti-MMP-9 antibodies and H-Score analysis carried out and migration analyzed by woundhealing, morphologically.
Results andConclusions:It’s been observed that boric acid doesn’t affect the EMT capability of the MCF-7 cells in terms of E-Cadherin and vimentin expression; whereas, it’s affect migration both by decreasing the MMP-9 expression and also by inhibiting migration.