Open AccessStatistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology
Author(s) -
Abd-El Aziem Farouk,
Anis Shobirin Meor Hussin,
Ralf Greiner,
Shareef M. Ismail,
Hamadah Mohd Nur Lubis
Publication year - 2017
Publication title -
asean journal on science and technology for development/asean journal on science and technology for development
Language(s) - English
Resource type - Journals
eISSN - 2224-9028
pISSN - 0217-5460
DOI - 10.29037/ajstd.304
Subject(s) - response surface methodology , central composite design , phytase , recombinant dna , incubation , arabinose , factorial experiment , escherichia coli , incubation period , chemistry , yield (engineering) , fractional factorial design , biology , microbiology and biotechnology , food science , biochemistry , chromatography , mathematics , enzyme , materials science , fermentation , statistics , xylose , metallurgy , gene
The production of phytase in a recombinant E.coli using the pBAD expression system was optimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentration in the cultivation media and the incubation temperature were optimized in order to maximize phytase production using 2 3 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among these factors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. Optimal conditions for phytase production were determined to be 100 µg/ml ampicilin, 0.2 % arabinose and an incubation temperature of 37ºC. The production of phytase in the recombinant E. coli was scaled up to 100 ml and 1000 ml.