Open AccessR unning Head: Novel Recombinant Phytate - d egrading Enzyme Production Statistical Optimization of the Induction of Phytase Production by Arabinose in a recombinant E. coli using Response Surface Methodology
Author(s) -
Abd. Elaziem Farouk .,
Anis Shobirin Meor Hussin,
Ralf Greiner,
Shareef M. Ismail,
Mohamed Faizal Noor Batcha,
Hamadah Mohd Nur Lubis
Publication year - 2017
Publication title -
asean journal on science and technology for development/asean journal on science and technology for development
Language(s) - English
Resource type - Journals
eISSN - 2224-9028
pISSN - 0217-5460
DOI - 10.29037/ajstd.179
Subject(s) - phytase , central composite design , response surface methodology , recombinant dna , arabinose , incubation , chemistry , factorial experiment , biochemistry , enzyme , biology , food science , fermentation , mathematics , chromatography , xylose , gene , statistics
The production of phytase in a recombinant E.coliusing thepBAD expression system wasoptimized using response surface methodology with full-factorial faced centered central composite design. The ampicilin and arabinose concentrationin the cultivation media and the incubation temperature were optimized in order to maximize phytase productionusing 23 central composite experimental design. With this design the number of actual experiment performed could be reduced while allowing eludidation of possible interactions among thesefactors. The most significant parameter was shown to be the linear and quadratic effect of the incubation temperature. OPtim al conditionsfor phytase production were determinedto be100 μg/ml ampicilin, 0.2 % arabinose and an incubation temperature of37ºC.The production of phytase in the recombinant E. coliwas scaledup to 100ml and 1000ml.