Open AccessEnzymatic Activity of Protease Producing Bacteria from Tofu Waste
Author(s) -
Isma Kurniatanty,
Esti Widowati
Publication year - 2021
Publication title -
the epic series in biological sciences
Language(s) - English
Resource type - Conference proceedings
ISSN - 2398-5291
DOI - 10.29007/j3ld
Subject(s) - protease , casein , food science , bacteria , microbiology and biotechnology , enzyme , biology , chemistry , biochemistry , genetics
Protease is an enzyme with high economic value and has been used in industries. Much natural waste can be considered as promising sources protease producing bacteria, such as tofu waste. The objective of the present study was to screen and isolate protease producing bacteria from tofu waste collected from Yogyakarta. Tofu waste samples were serially diluted and 0.1 ml of sample was spread on skim milk agar, at 37oC for 48 hours. Four bacterial colonies showed a clear zone around the colony, indicating protease activity. These isolates (encoded as TWB-1, TWB-2, TWB-3, and TWB-4) were subjected to several biochemical tests and gram staining. Protease activity was determined using the tyrosine standard curve with the casein-Hammarsten substrate. Protease activity of TWB-1, TWB-2, TWB-3, and TWB-4 were 2.85, 5.74, 5.14, and 3.00 respectively. Isolate TWB-2 showed the maximum protease activity with 5.74 U/ml.