Open AccessAnalysis of differential expression of lipid metabolism genes in atherosclerotic plaques in patients with coronary atherosclerosis
Author(s) -
Elena Shakhtshneider,
D. Ivanoshchuk,
Yu. I. Ragino,
Veniamin Fishman,
Yа. V. Polonskaya,
Е. В. Каштанова,
Alexander Cherniavsky,
И. С. Мурашов,
М. И. Воевода
Publication year - 2022
Publication title -
sibirskij žurnal kliničeskoj i èksperimentalʹnoj mediciny
Language(s) - English
Resource type - Journals
eISSN - 2713-2927
pISSN - 2713-265X
DOI - 10.29001/2073-8552-2021-36-4-156-163
Subject(s) - coronary atherosclerosis , medicine , apolipoprotein b , lipid metabolism , arteriosclerosis , gene , apolipoprotein e , gene expression , pathology , cardiology , cholesterol , coronary artery disease , biology , disease , genetics
Aim. The goal of the study was to analyze the differential expression of lipid metabolism-related genes in the atherosclerotic plaques of different types in patients with coronary atherosclerosis. Material and Methods. The study was performed on the specimens of atherosclerotic plaques in 45–65-year-old patients with coronary atherosclerosis with stable exertional angina functional class II-IV without acute coronary syndrome. Coronary atherosclerosis was verified by coronary angiography. Atherosclerotic plaque tissue was sampled intraoperatively when indicated. Whole-genome sequencing of ribonucleic acid (RNA) was performed using the TruSeq RNA Sample Preparation Kit (Illumina, USA). Results. We analyzed the differences in the expression of 12 genes including LDLR, APOB, PCSK9, LDLRAP1, LIPA, STAP1, ABCA1, APOA1, APOE, LPL, SCARB1 , and SREBF2 depending on the type of atherosclerotic plaques. The expression level of APOE gene was eight times higher in unstable atherosclerotic plaques of dystrophic-necrotic type ( p < 0.0001). The expression levels of LDLR and APOB genes were eight times higher in stable atherosclerotic plaques ( p < 0.0001). We did not find differences in the expression levels of the ABCG5, ABCG8, APOC3, CETP, CLPS, CYP7A1 , and PNPLA5 genes. Conclusion. The study showed the differences in the activity of individual metabolism-related genes in the atherosclerotic plaques of different types in patients with coronary atherosclerosis. Obtained data may become the basis for the development of test systems aimed at predicting the development of atherosclerotic process and its complications.