Open AccessPolymorphism of DNA-microsatellites of Ayrshire cattle of the Komi Republic and the Kirov region
Author(s) -
S.V. Nikolaev
Publication year - 2021
Publication title -
agrarnyj naučnyj žurnal
Language(s) - English
Resource type - Journals
eISSN - 2587-9944
pISSN - 2313-8432
DOI - 10.28983/asj.y2021i9pp67-70
Subject(s) - locus (genetics) , microsatellite , biology , allele , loss of heterozygosity , population , genetics , allele frequency , gene , demography , sociology
The allelofund of Ayrshire cattle of the Komi Republic (n=340) and the Kirov region (n=32) was evaluated using microsatellite DNA markers. It was found that in the studied populations, the TGLA227 locus was the most polymorphic – 9 alleles, and the BM1824 and TGLA126 loci had the least alleles (3-4). The allele of 214 loci INRA23 (q=0.7387) was found with the highest frequency in Ayrshire cattle in the Komi Republic, and 115 loci TGLA126 (q=0.7083) was found among animals of the Kirov region. The average number of alleles per locus in the samples of the Komi Republic was slightly higher (6.6±0.5), compared to the indicator in cows of the Kirov region (6.2±0.4). The number of effective alleles in the Komi population was 3.2±1.0, and in the animals of the Kirov region-3.4±1.0. The actual heterozygosity at all loci in animals of the Komi Republic was lower by 22.4% (P<0.001). The highest homozygosity in cows of the Republic was observed for loci INRA23 (Ho=0.449) and SPS115 (Ho=0.579), and in the sample of animals of the Kirov region for TGLA126 (Ho=0.771) and INRA23 (Ho=0.875). The fixation index in Ayrshire cattle of the Kirov region was -0.273±0.055, which is 6.8 times higher (P <0.001) compared to the Komi population (Fis= -0.040±0.015).