Open AccessEffect of silicon dimethylglycerolate on ripeing of sheep oocytes in vitro
Author(s) -
Tatyana Ivanovna Stanislavovich
Publication year - 2020
Publication title -
agrarnyj naučnyj žurnal
Language(s) - English
Resource type - Journals
eISSN - 2587-9944
pISSN - 2313-8432
DOI - 10.28983/asj.y2020i11pp99-101
Subject(s) - andrology , in vitro , in vitro maturation , oocyte , silicon , meiosis , biology , chemistry , microbiology and biotechnology , embryo , medicine , biochemistry , gene , organic chemistry
In the presented study, a positive effect of a silicon-containing compound, silicon dimethylglycerolate (SDMG), on cells of cumulus expansion and nuclear maturation of sheep oocytes was established. This chemical compound is characterized by wound healing, regenerating and transmucosal activity. The introduction of 0.2% SDMG into the composition of the culture medium as an additional component increased the level of oocytes with expanded cumulus (33% versus 21%, P <0.05). The yield of oocytes that reached the final stage of meiotic maturation was 88% under the conditions of using SDMG versus 71% (P <0.05) in the control group. The results obtained suggest the possibility of using SDMG in the technology of extracorporeal maturation of donor sheep oocytes.