Open AccessEvaluation of Oxidative Stress as a Contributor of Anesthetic Agents' Effects on Embryo Quality and ICSI Outcome in Iraqi Patients
Author(s) -
Athraa Abd Alhussain,
Raad Ghazi Reshan,
Hayder A. L. Mossa
Publication year - 2021
Publication title -
iraqi journal of embryos and infertility researches
Language(s) - English
Resource type - Journals
eISSN - 2616-6984
pISSN - 2218-0265
DOI - 10.28969/ijeir.v10.i2.r2
Subject(s) - remifentanil , follicular fluid , medicine , ketamine , intracytoplasmic sperm injection , oocyte , anesthetic , embryo quality , embryo transfer , anesthesia , in vitro fertilisation , embryo , biology , propofol , microbiology and biotechnology
Infertility is described as a couple's failure to conceive for at least a year, using unprotected sex. Even though all anesthetic chemicals have been revealed in the follicular fluids, general anesthesia is still used in many IVF centers for patients who want to get pregnant. This study included 60 infertile women randomly divided into two groups under general anesthesia. 30 patients were given a Ketamine dose of 0.5 mg/Kg and the other 30 patients were received Remifentanil dose of 0.5 µg/Kg for induction of anesthesia. We collected serum for Reactive Oxygen Species (ROS) levels assessment for all patients before and after starting general anesthesia. Routine ICSI procedures was performed on all participants, including clinical evaluation (history, examination, and investigation), controlled ovarian and ovulation stimulation, oocyte retrieval under general anesthesia, follicular fluid collection for postoperative anesthesia medication concentration (Remifentanil and Ketamine), oocyte stripping, oocyte maturity assessment, intra-oocyte sperm injection into mature cells (MII), fertilization and division evaluation and embryo categorization, embryo selection and transfer, luteal phase support, and beta-hCG determination. ROS concentrations were compared between the Remifentanil and Ketamine groups. There was no significant difference in embryo features between Remifentanil and Ketamine, indicating that neither one is superior to the other in this regard. When Remifentanil or Ketamine was taken, there was no significant difference in ROS levels in serum or follicular fluid.