Open AccessIMPROVEMENT METHOD OF GENE TRANSFER IN Kappaphycus alvarezii
Author(s) -
St. Hidayah Triana,
Alimuddin Ali,
Utut Widyastuti,
. Suharsono,
Emma Suryati,
Andi Parenrengi
Publication year - 2016
Publication title -
e-journal ilmu dan teknologi kelautan tropis
Language(s) - English
Resource type - Journals
ISSN - 2085-6695
DOI - 10.28930/jitkt.v8i1.13087
Subject(s) - span (engineering) , biology , zoology , horticulture , structural engineering , engineering
Method of foreign gene transfer in red seaweed Kappaphycusalvarezii has been reported, however, li-mited number of transgenic F 0 (broodstock) was obtained. This studywas conducted to improve the method of gene transfer mediated by Agrobacteriumtumefaciens in order to obtain high percentage of K. alvarezii transgenic. Superoxide dismutase gene from Melastomamalabatrichum (MmCu/Zn-SOD) was used as model towards increasing adaptability of K. alvarezii to environmental stress. The treat-ments were the culture media and recovery duration, and each treatment consisted of three replica-tions. The best method was co-cultivation using liquid media, then recovery was conducted in liquidmedia for10days. That treatment allowed higher transformation percentage ( 90% ) , regeneration effi-ciency ( 90% ) , putative bud efficiency (100%) , number of buds and explants sprouted (100%) and transgenic explants ( 100% ) . The transgenic explants showed an amplification PCR product of Mm-Cu/Zn-SOD gene fragment , whereas the non-transgenic explants showed no amplification product. All results revealed that suitable method of transgenesis for K. alvarezii has been developed.Keywords : Agrobacterium tumefaciens , culture media, Kappaphycus alvarezii , recovery duration, transformation