Open AccessBACTERIAL SYMBIONT BIOACTIVE COMPOUND OF SOFT CORAL Sinularia flexibilis AND S. polydactyla
Author(s) -
Rozirwan Rozirwan,
Dietriech G. Bengen,
. Chaidir,
Neviaty P. Zamani,
Hefni Effendi
Publication year - 2016
Publication title -
e-journal ilmu dan teknologi kelautan tropis
Language(s) - English
Resource type - Journals
ISSN - 2085-6695
DOI - 10.28930/jitkt.v7i2.10994
Subject(s) - span (engineering) , life span , style (visual arts) , biology , evolutionary biology , art , literature , engineering , structural engineering
Symbiont bacteria on soft coral can produce bioactive compounds that plays an important role in chemical ecology and as a marine natural product. The purpose of this study was to find and characterize the antibacterial activities of active compounds extracted from bacterial symbionts of soft coral S . flexibilis and S . polydactyla . The methods used in this study were culture and isolation of bacterial symbionts, extraction of compounds, antibacterial bioassay, and identification of bioactive compounds using the LC-MS analyses. Four isolates of bacterial symbionts were obtained from two samples of soft corals, 2 isolates of Pseudomonas diminuta (A1) and Edwardsiellla hoshinae (A2) from soft coral S. flexibilis , and 2 isolates of E. hoshinae (B1) and P . acidovorans (B4) from S. polydactila . Antibacterial activity were found only from the extracts of bacterial symbionts P. diminuta (A1) and from S . flexiblis about 10.16 ± 0.3mm (for B . subtilis ), 8.66 ± 0.8 mm ( E. coli ) and 9.86 ± 1.7mm ( S . dysentri ). No antibacterial activity found from the extracts of S . polydactyla . The results of LC MS analysis showed that the group of diterpenes sinularin produced by soft corals S . flexibilis and bacterial symbionts isolates of P . diminuta (A1).Keywords: Bacterial Symbiont, Bioactive Compound, Antibacterial Activity, Soft Coral, Sinularia flexibilis, Sinularia polydactyla