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Effects of platelet rich plasma and acellular bone marrow on gene expression patterns and DNA content of equine suspensory ligament explant cultures
Author(s) -
SCHNABEL L. V.,
MOHAMMED H. O.,
JACOBSON M. S.,
FORTIER L. A.
Publication year - 2008
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.2746/042516408x278030
Subject(s) - decorin , platelet rich plasma , bone marrow , anabolism , explant culture , matrix metalloproteinase , andrology , chemistry , cartilage , microbiology and biotechnology , platelet , medicine , immunology , biology , anatomy , in vitro , biochemistry , proteoglycan
Summary Reasons for performing study : Suspensory ligament (SL) desmitis is a common source of lameness. The results of this study will determine if blood‐derived products stimulate SL matrix synthesis and have potential as regenerative therapies for SL desmitis Objectives : To determine if various blood‐based biological products including plasma, blood, PRP, platelet poor plasma (PPP) and ABM aspirate stimulates anabolic and/or catabolic pathways in suspensory ligaments (SL). Methods : The body of the SL was harvested from 6 horses and used to establish explant cultures. Explants were cultured in plasma, blood, PRP, PPP or ABM at concentrations of 10, 50 or 100%. Anabolic responses were assessed by use of quantitative PCR for collagens type I and III , cartilage oligomeric matrix protein (COMP) and decorin. Total DNA and collagen protein content were also measured. Catabolic reactions were measured by quantitative PCR for matrix metalloproteinases 3 and 13 (MMP‐3, MMP‐13). Results : Acellular bone marrow aspirate at 100% stimulated decorin and COMP mRNA synthesis more than all other treatments at all concentrations. No treatment at any concentration stimulated the catabolic gene MMP‐13; only 50% ABM stimulated MMP‐13 mRNA expression. Conclusions : Acellular bone marrow is indicated, and might be preferred to plasma, blood or PPP, as a blood‐based biological source for SL tissue regenerative therapy. Long‐term, placebo controlled case studies are indicated to determine if ABM aids in recovery from SL desmitis. Potential relevance : Bone marrow aspirate is an autogenous, readily available biological source for SL regenerative therapy where the aim is to stimulate matrix synthesis.