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Comparison of the antioxidant status in tracheal and bronchoalveolar epithelial lining fluids in recurrent airway obstruction
Author(s) -
DEATON C. M.,
MARLIN D. J.,
DEATON L.,
SMITH N. C.,
HARRIS P. A.,
SCHROTER R. C.,
KELLY F. J.
Publication year - 2006
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.2746/042516406778400556
Subject(s) - ascorbic acid , bronchoalveolar lavage , medicine , antioxidant , inflammation , lung , oxidative stress , pathology , respiratory system , immunology , chemistry , biochemistry , food science
Summary Reasons for performing study : Following a period of airway inflammation the clearance of inflammatory cells along the mucociliary escalator may impose a considerable oxidant load on the trachea. Objectives : To determine the degree of oxidative stress in tracheal epithelial lining fluid (ELF) in comparison to that present in peripheral airways after an acute exposure to organic dust. Methods : Tracheal wash fluid and bronchoalveolar lavage fluid (BALF) were collected for cytology and antioxidant analyses from 6 recurrent airway obstruction (RAO)‐affected horses and 6 healthy control horses before and after stabling on straw bedding for 24 h. Results : In RAO‐affected horses, organic dust exposure resulted in a significant decrease in ascorbic acid concentration in tracheal ELF (P < 0.0001), which was greater than the decrease in bronchoalveolar ELF (P = 0.0003). The percentage decrease in tracheal ELF ascorbic acid correlated with the percentage decrease in bronchoalveolar ELF ascorbic acid (r = 0.76; P = 0.004) following exposure. Conclusions : Acute organic dust exposure results in significant antioxidant depletion in the trachea, which may reflect inflammation and oxidative processes in peripheral airways. Potential relevance : Further work is required to evaluate the role of ascorbic acid depletion in the pathogenesis of RAO.