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A dose titration of triamcinolone acetonide on insulin‐like growth factor‐1 and interleukin‐1‐conditioned equine cartilage explants
Author(s) -
Sandler E. A.,
Frisbie D. D.,
MCIlwraith C. W.
Publication year - 2004
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.2746/0425164044864615
Subject(s) - cartilage , triamcinolone acetonide , anabolism , interleukin , endocrinology , explant culture , medicine , chemistry , growth factor , glycosaminoglycan , in vivo , in vitro , pharmacology , immunology , biology , biochemistry , cytokine , anatomy , receptor , microbiology and biotechnology
Summary Reasons for performing study : Previous in vitro pilot studies have defined a potentially beneficial effect of insulin‐like growth factor‐1 (IGF‐1) and triamcinolone acetonide (TA) on interleukin‐1 (IL‐1)‐conditioned equine cartilage. Furthermore, an optimal dose for IGF‐1 treatment alone has been documented previously using the same test system as in the current project. Objectives : To perform a dose titration of TA on IL‐1‐conditioned equine articular cartilage explants in the presence of an optimised IGF‐1 dose, in order to optimise a triamcinolone concentration for use in combination with IGF‐1 for future investigations. Methods : Cartilage explants were harvested from the distal femur of a normal horse. The effect of a clinically relevant TA dose range was evaluated in the presence of IL‐1 and IGF‐1 through measurement of proteoglycan (PG) matrix metabolism (synthesis and degradation). Results : TA and IGF‐1 in combination inhibited the IL‐1‐induced release of PG matrix components (glycosaminoglycan or GAG) from the articular cartilage, as well as producing a significant increase in GAG synthesis. Conclusions : This experiment provided proof of principle that a combination treatment appears to be able to combat the IL‐1‐induced matrix depletion, while enhancing anabolic metabolism within the articular cartilage. Potential relevance : The use of IGF‐1 in conjunction with TA in vivo has the potential to provide beneficial anabolic effects not seen with TA alone.