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Detection of cold‐adapted vaccine‐strain influenza virus using two commercial assays
Author(s) -
ADAM E. N.,
MORLEY P. S.,
CHMIELEWSKI K. E.,
CARMAN J.,
GONZALES G.
Publication year - 2002
Publication title -
equine veterinary journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.82
H-Index - 87
eISSN - 2042-3306
pISSN - 0425-1644
DOI - 10.2746/042516402776249218
Subject(s) - virology , virus , horse , nasal administration , vaccination , equine influenza , medicine , antigen , population , influenza vaccine , influenza a virus , immunology , biology , paleontology , environmental health
Summary Because of the contagious nature of influenza virus it is necessary to identify infected individuals after the virus is introduced into a population. The aim of this study was to characterise influenza virus detection with commercially available assays after intranasal vaccinating horses with cold‐adapted influenza virus. Seven horses were vaccinated and placed with 3 unvaccinated horses. Nasal secretion samples were evaluated using 2 antigen detection assays. All 10 horses were positive in the Flu OIA assay during the study period, but only one horse was positive on one sample using the Directigen Flu A assay. Horses were most likely to be positive during the first 3 days following vaccination, and several horses were intermittently positive for several days after this. Obtaining positive test results from nonvaccinated, incontact horses suggests they became infected with vaccine‐strain virus that was shed by vaccinated horses. These results are important for the correct interpretation of influenza antigen detection tests in situations when this modified‐live intranasal vaccine has been used.