Open AccessDesenvolvimento de método espectrofotométrico ambientalmente mais limpo para análise de nitrito em salsichas
Author(s) -
Ângela Pinheiro Martins,
Vitor Hugo Marques Luiz,
Leonardo Pezza,
Helena Redigolo Pezza
Publication year - 2016
Publication title -
eclética química
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.177
H-Index - 19
eISSN - 1678-4618
pISSN - 0100-4670
DOI - 10.26850/1678-4618eqj.v40.1.2015.p133-140
Subject(s) - physics , humanities , philosophy
A clean environmental spectrophotometric method for the analysis of nitrite using a new diazotizing reagent is reported. The proposed method is based on diazotization-coupling reaction between dapsone, nitrite and (naphthyl)ethylenediamine (NED) in phosphoric acid medium at room temperature. Experimental design methodologies were used to optimize the measurement conditions. The spectrophotometric measurements were carried out at 545 nm and the linear range obtained was 5.0 x 10-3 mg.L-1 – 0.5 mg.L-1, with detection and quantification limits of 0.99 x 10-3 mg.L-1 e 3.31 x 10-3 mg.L-1, respectively. The proposed method was applied successfully to the analysis of nitrite in sausages and the results obtained by the proposed method were in good agreement with those obtained using the official method.